Accuracy of the analysis of free vancomycin concentration by ultrafiltration in various disease states

作者：Zhang, Jin-feng; Yang, Xiu-ling; Zhang, Zhi-qing; Dong, Wei-chong; Jiang, Ye^*

来源：RSC Advances, 2014, 4(76): 40214-40222.

DOI：10.1039/c4ra06575a

• COLLOID OSMOTIC-PRESSURE
• SERUM-PROTEIN BINDING
• LIQUID-CHROMATOGRAPHY
• MYCOPHENOLIC-ACID
• FREE DRUG
• PLASMA
• VOLUME

In the present study, the accuracy of the analysis of free vancomycin (VCM) by ultrafiltration in various disease conditions was assessed. With VCM as a representative drug, we used clinical plasma samples to investigate the effect of plasma conditions on the volume ratio of ultrafiltrate to sample solution (V-u/V-s) and the consequential effect on the measured free drug concentration (f(c)). Our results demonstrated that plasma conditions had a significant impact on V-u/V-s by centrifugal ultrafiltration (CF-UF). The V-u by CF-UF ranged from 97 mu L to 279 mu L among different individuals under the same centrifugation conditions. Total protein levels and the osmotic pressure of plasma were the main influence factors of V-u/V-s in disease states. In contrast, the V-u/V-s by hollow fiber centrifugal ultrafiltration (HFCF-UF) was less influenced by plasma conditions. As a consequence, the results of f(c) determined by HFCF-UF were more accurate than those by CF-UF for patients with different disease states. HFCF-UF dis

• 出版日期2014
• 单位河北医科大学