Application of ionic liquids for elution of bioactive flavonoid glycosides from lime fruit by miniaturized matrix solid-phase dispersion

作者：Xu, Jing-Jing; Yang, Rui; Ye, Li-Hong; Cao, Jun^*; Cao, Wan; Hu, Shuai-Shuai; Peng, Li-Qing

来源：Food Chemistry, 2016, 204: 167-175.

DOI：10.1016/j.foodchem.2016.02.012

• Flavonoid glycosides
• Lime fruit
• Ionic liquids
• Miniaturized matrix solid-phase dispersion
• Ultra-performance liquid chromatography

In this work, two flavonoid glycosides (neohesperidin and naringin) in lime fruit were effectively extracted by miniaturized matrix solid phase dispersion (MSPD), followed by ultra-performance liquid chromatography-ultraviolet detection. The best results were obtained using Florisil (150 mg) as the sorbent and 1-butyl-3-methylimidazolium tetrafluoroborate (0.4 mL, 250 mM) as the elution solvent. This work represents the first attempt of using ionic liquids as a green eluent for extraction of the investigated compounds in miniaturized MSPD. Compared with the conventional methods, the proposed method is advantageous due to improved enrichment factor and reduced reagent consumption. A good linearity was observed with r(2) values (>0.998). Meanwhile, the method gave acceptable recoveries (90.16-96.47%) for the determination of flavonoids in plant samples. The limits of detection of the two analytes ranged between 4.08 and 5.04 mu g/g. The results showed that the optimized method has a great potential for sample preparation in routine analysis of complex plant samples.

• 出版日期2016-8-1
• 单位杭州师范大学