Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone

作者：Im Hyungsoon; Castro Cesar M; Shao Huilin; Liong Monty; Song Jun; Pathania Divya; Fexon Lioubov; Min Changwook; Avila Wallace Maria; Zurkiya Omar; Rho Junsung; Magaoay Brady; Tambouret Rosemary H; Pivovarov Misha; Weissleder Ralph^*; Lee Hakho

来源：Proceedings of the National Academy of Sciences, 2015, 112(18): 5613-5618.

DOI：10.1073/pnas.1501815112

• cancer diagnostics
• smartphone
• molecular sensing
• point-of-care diagnostics
• cervical cancer

The widespread distribution of smartphones, with their integrated sensors and communication capabilities, makes them an ideal platform for point-of-care (POC) diagnosis, especially in resource-limited settings. Molecular diagnostics, however, have been difficult to implement in smartphones. We herein report a diffraction-based approach that enables molecular and cellular diagnostics. The D3 (digital diffraction diagnosis) system uses microbeads to generate unique diffraction patterns which can be acquired by smartphones and processed by a remote server. We applied the D3 platform to screen for precancerous or cancerous cells in cervical specimens and to detect human papillomavirus (HPV) DNA. The D3 assay generated readouts within 45 min and showed excellent agreement with gold-standard pathology or HPV testing, respectively. This approach could have favorable global health applications where medical access is limited or when pathology bottlenecks challenge prompt diagnostic readouts.

• 出版日期2015-5-5