Nature's recipe for splitting inteins

作者：Aranko A Sesilja; Wlodawer Alexander; Iwai Hideo^*

来源：Protein Engineering Design and Selection, 2014, 27(8): 263-271.

DOI：10.1093/protein/gzu028

• protein ligation
• protein splicing
• protein transsplicing
• split intein

Protein splicing in trans by split inteins has increasingly become a powerful protein-engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring and artificially engineered split inteins have been reported for protein ligation using protein trans-splicing. Here, we review the current status of the reported split inteins in order to delineate an empirical or rational strategy for constructing new split inteins suitable for various applications in biotechnology and chemical biology.

• 出版日期2014-8