摘要

Epoxidized dialdehyde cellulose (EDC) was prepared and grafted with melamine to obtain melamine grafted epoxidized dialdehyde cellulose (EDC-melamine); the products were characterized by various methods and were used as carriers to immobilize laccase. Results show EDC-melamine can immobilize laccase effectively and have higher enzymatic activity compared with EDC. Furthermore, the enzymatic activity of EDC-melamine was found to be as high as 865 U center dot mg(-1), compared with 140U center dot mg(-1) for EDC. The removal efficiency of 2,4-dichlorophenol (2,4-DCP) for EDC- melamine immobilized laccase was about 71.5% at 40 degrees C for 4 h at 10.0 mg center dot L-1 and dosage of laccase = 0.2 g/L. The removal efficiency can remain greater than 63%, even after six cycles.