Dot immunogold filtration assay (DIGFA) for the rapid detection of specific antibodies against the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) using purified 31-kDa antigen

作者：Eamsobhana P^*; Gan X X; Ma A; Wang Y; Wanachiwanawin D; Yong H S

来源：Journal of Helminthology, 2014, 88(4): 396-401.

DOI：10.1017/S0022149X13000321

A rapid dot immunogold filtration assay (DIGFA) was adopted for specific immunodiagnosis of human cerebral angiostrongyliasis, using purified 31-kDa glycoprotein specific to Angiostrongylus cantonensis as diagnostic antigen and protein A colloidal gold conjugate as antigen-antibody detector. A total of 59 serum samples were assayed-11 samples from clinically diagnosed patients with detectable A. cantonensis-specific antibody in immunoblotting; 23 samples from patients with other related parasitic diseases, i.e. gnathostomiasis (n = 8), cysticercosis (n = 5), toxocariasis (n = 2), filariasis (n = 4), paragonimiasis (n = 2) and malaria (n = 2); and 25 samples from normal healthy subjects. The sensitivity and specificity of DIGFA to detect anti-A. cantonensis specific antibodies in serologically confirmed angiostrongyliasis cases, were both 100%. No positive DIGFA was observed in cases with other parasitic diseases, and the healthy control subjects. The 3-min DIGFA is as sensitive and specific as the 3-h immunoblot test in angiostrongyliasis confirmed cases that revealed a 31-kDa reactive band. The gold-based DIGFA is more rapid and easier to perform than the traditional enzyme-linked immunosorbent assay (ELISA). The test utilizing purified A. cantonensis antigen is reliable and reproducible for specific immunodiagnosis of human infection with A. cantonensis-thus can be applied as an additional routine test for clinical diagnostic support. Large-scale seroepidemiological studies in endemic communities in north-east Thailand are under way to evaluate its usefulness under field conditions.

• 出版日期2014-12
• 单位浙江省医学科学院