A novel type-1 cytokine receptor from fish involved in the Janus kinase/signal transducers and activators of transcription (Jak/STAT) signal pathway

作者：Santos Mudjekeewis D; Yasuike Motoshige; Kondo Hidehiro; Hirono Ikuo; Aoki Takashi^*

来源：Molecular Immunology, 2007, 44(13): 3355-3363.

DOI：10.1016/j.molimm.2007.02.018

• Japanese flounder (Paralichthys olivaceus)
• type-1 cytokine receptor
• novel
• poly I : C
• Jak/STAT signal pathway
• immunity
• development

Type I cytokine receptors mediate the action of the members of the long chain cytokines canonically involved in numerous physiological function. Here we report a novel cytokine receptor termed Japanese flounder glycoprotein 130 homologue or JfGPH, exhibiting the unique type I cytokine receptor motifs i.e. having a cytokine binding domain (CBD) containing two pairs of conserved cysteine (C) residues. a WSXWS motif, three fibronectin domains all in the extracellular region. It is also composed of the Jak binding domains Box I and Box 2, and a STAT 3 binding motif (Box 3) in the cytoplasmic region suggesting its mediatory role for Janus kinase/Signal transducers and activators of transcription (Jak/STAT) signal pathway. The JfGPH cDNA is about 3 kb encoding 801 amino acid residues with a predicted molecular weight of 90 kDa and its gene has an 11-exon/10-intron architecture. While JfGPH shows significant homology with the members of type-1 cytokine receptor family including IL6ST (or gp 130), IL31 alpha (or GLMR), CSF3R (or GCSFR), LIFR, OSMR, IL12R beta 1 and LEPR, structural and phylogenetic analysis of its protein revealed that it is a novel and an-ancestral cytokine receptor found in teleost. JfGPH gene is ubiquitously expressed in Japanese flounder tissues and in a natural embryo (HINAE) cell line showing its critical role in teleost physiological functions similar to gp 130 in higher vertebrates. High expression of JfGPH transcripts in immune-related tissues and, in ovary and embryo-derived cell line suggest its role in immune responses, and reproduction/development, respectively. In vitro stimulation of spleen, kidney, peripheral blood leukocytes (PBLs) and HINAE revealed that JfGPH is down-regulated by polyinosinic:polycytidylic acid (poly I:C), an interferon (IFN) inducer, suggesting an apparent control of the JfGPH's expression during IFN-induced Jak/STAT signaling.

• 出版日期2007-7