Upregulation of miR-132 attenuates osteoblast differentiation of UC-MSCs

作者：Xue, Z. -L.; Meng, Y. -L.; Ge, J. -H.^*

来源：European Review for Medical and Pharmacological Sciences, 2018, 22(6): 1580-1587.

• miR-132
• Wnt/beta-catenin
• Osterix
• UC-MSCs
• Osteoblast

OBJECTIVE: Wnt/beta-catenin signaling pathway plays a role in upregulating expression of osteoblast (OB) specific transcriptional factor Osterix and promoting OB differentiation. It was shown that the elevation of the miR-132 level was associated with sclerotizing inhibition. Bioinformatics analysis revealed the complementary binding site between miR-132 and 3'-UTR of beta-catenin. This study investigated the influence of miR-214 in regulating beta-catenin expression and differentiation of umbilical cord mesenchymal stem cells (UC-MSCs) into OB. @@@ MATERIALS AND METHODS: UC-MSCs were induced to differentiate to OB. The expressions of miR-132, beta-catenin, Osterix, and ALP, together with ALP activity were detected on day 0, 5, 10, and 15. The regulatory relationship between miR-132 and beta-catenin was confirmed by dual luciferase reporter gene assay. UC-MSCs were divided into five groups, including agomir-control, miR-132 agomir, pGPH1-NC, pGPH1-beta-catenin, and miR-132 agomir + pGPH1-beta-catenin groups. beta-catenin, Osterix, and ALP expressions, together with ALP activity were tested after induction for 15 days. @@@ RESULTS: MiR-132 was downregulated, while p-catenin Osterix and ALP expressions, together with ALP activity were enhanced in the process of UC-MSCs differentiating into OBs. MiR-132 agomir and/or pGPH1-beta-catenin transfection significantly reduced beta-catenin expression, downregulated Wnt/beta-catenin signaling pathway activity, declined Osterix level, weakened ALP expression and activity, and attenuated OB differentiation of UC-MSCs. @@@ CONCLUSIONS: The level of beta-catenin was enhanced, while the miR-132 level was decreased in the process of UC-MSCs differentiating into OBs. Upregulation of miR-132 inhibited the differentiation of UC-MSCs through suppressing beta-catenin expression, attenuating Wnt/beta-catenin signaling pathway activity, and downregulating Osterix level.

• 出版日期2018-3
• 单位西南医科大学