A reliable, non-invasive PCR method for giant panda (Ailuropoda melanoleuca) sex identification

作者：Xu Xiao; Lin Ling; Zhang Zhihe; Shen Fujun; Zhang Liang; Yue Bisong^*

来源：Conservation Genetics, 2008, 9(3): 739-741.

DOI：10.1007/s10592-007-9376-1

• amelogenin gene
• giant panda
• non-invasive technique
• sex identification
• ursidae

We developed an inexpensive, fast and reliable PCR method for sex identification of giant panda (Ailuropoda melanoleuca) by using one pair of primers to co-amplify homologous fragments with size polymorphism that located at amelogenin (AMEL) exon 5. In giant panda, a 63 bp deletion in exon 5 of Y-linked allele provides a significant discrimination between AMELX and AMELY, thus the amplification products can be distinguished simply by agarose gel electrophoresis, exhibiting sex-specific banding patterns (male: 237 bp, 174 bp; female: 237 bp). Both blood and feces samples from known-sex giant pandas were successfully amplified. Cross species test also revealed that this method could be applied to other Ursidae species.

• 出版日期2008-6
• 单位成都大熊猫繁育研究基地; 四川大学