Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3

作者：Yu Leiye; Sun Guojie; Wei Jingfang; Wang Yingze; Du Chao; Li Jiang^*

来源：Chinese Journal of Oceanology and Limnology, 2016, 34(5): 1064-1071.

DOI：10.1007/s00343-016-4393-x

• exopolysaccharide (EPS)
• Antarctic bacterium
• RAW264.7
• TLR4
• NF-kappa B

An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF) a secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and I kappa B alpha degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-kappa B signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.

• 出版日期2016-9
• 单位国家海洋局第一海洋研究所; 河北科技大学