A Nucleoporin. Docks Protein Phosphatase to Direct Meiotic Chromosome Segregation and Nuclear Assembly

作者：Hattersley Neil; Cheerambathur Dhanya; Moyle Mark; Stefanutti Marine; Richardson Amelia; Lee Kian Yong; Dumont Julien; Oegema Karen; Desai Arshad^*

来源：Developmental Cell, 2016, 38(5): 463-477.

DOI：10.1016/j.devcel.2016.08.006

During M-phase entry in metazoans with open mitosis, the concerted action of mitotic kinases disassembles nuclei and promotes assembly of kinetochores the primary microtubule attachment sites on chromosomes. At M-phase exit, these major changes in cellular architecture must be reversed. Here, we show that the conserved kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of protein phosphatase 1 (PP1c) to direct kinetochore disassembly-dependent chromosome segregation during oocyte meiosis I and nuclear assembly during the transition from M phase to inter phase. During oocyte meiosis I, MEL-28-PP1c disassembles kinetochores in a timely manner to promote elongation of the acentrosomal spindles that segregate homologous chromosomes. During nuclear assembly, MEL-28 recruits PP1c to the periphery of decondensed chromatin, where it directs formation of a functional nuclear compartment. Thus, a pool of phosphatase activity associated with a kinetochore-localized nucleoporin contributes to two key events that occur during M-phase exit in metazoans: kinetochore disassembly and nuclear reassembly.

• 出版日期2016-9-12