科研之友
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摘要
Based on the biosynthetic pathway of firefly bioluminescence substrate D-luciferin, the concentration of L-Cysteine can be quantified using a simple protocol and a conventional luminescence detector. The lower limit of quantification (signal/noise ratio [S/N] = 10) was 0.26 mu M. Using our method, the total amount of free/reduced and disulfide/oxidized L-cysteine could be measured successfully in human serum. In addition, biosynthetic precursors such as 2-cyano-6-hydroxybenzothiazole and L-luciferin could replace D-luciferin in the cell-based luciferase assay. Our results suggest that the bioluminescence reaction associated with the biosynthesis of bioluminescence substrates can provide a fast and cost-effective assay method.
- 出版日期2010-1-15
