Analysis of Peptide Mixtures for Proteomics Research Using LC-ESI-MS with a Simple Microgradient Device

作者：Lenobel Rene; Rehulkova Helena; Sebela Marek; Franc Vojtech; Kahle Vladislav; Moravcova Dana; Rehulka Pavel^*

来源：LC-GC North America, 2015, 33(6): 420-+.

This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography (LC) and electrospray ionization (ESI) tandem mass spectrometry (MS-MS). The formation of an S-shaped gradient of the strong mobile phase (based on acetonitrile) inside a microsyringe is described under the conditions applied for capillary separation of proteomic samples and their analysis using ESI-MS-MS on a hybrid instrument. The system was successfully tested for a complex peptide mixture originating from the digestion of the whole-cell lysate from Francisella tularensis subsp. Schu 54 and it represents a cheap alternative for the separation of both simple and complex peptide mixtures before MS analysis.

• 出版日期2015-6