科研之友
微信
新浪微博
Facebook
分享链接
摘要
To establish the method for qualitative and quantitative determination of gallic acid in Herba Gei. Gallic acid content is determined by HPLC with Diamonsil C-18 column as the stationary phase, under the conditions of methanol-0.1% phosphoric acid in water (10: 90) mobile phase, 271 nm detection wavelength, and 40 degrees C column temperature. Peak area of gallic acid shows a good linearity with the injection volume within a 0.202 similar to 1.212 mu g range, r=0.9997; average recovery is 99.26%, RSD 2.20%. Through quantitative determination of Herba Gei of different origins, the method established herein is confirmed to be simple, fast, sensitive, accurate, reliable and reproducible.
- 出版日期2015-6
- 单位西南医科大学
