A novel mitochondria-targeting fluorescent probe for hydrogen sulfide in living cells

作者：Shi, Wei; Pan, Miaobo; Qiang, Hao; Qiu, Qianqian; Huang, Wenlong; Lin, Haiyan; Qian, Hai^*; Ge, Liang^*

来源：Chemical Biology & Drug Design, 2017, 90(2): 167-174.

DOI：10.1111/cbdd.12948

• fluorescent probe
• hydrogen sulfide
• mitochondria targeting
• selectively

A novel mitochondria-targeting fluorescent probe compound S-N-3 for hydrogen sulfide (H2S) in living cells has been designed and synthesized in this study. This article contained the chemosynthesis and some studies on bioactivity of the target compound in living cells. Compound S-N-3 is easy to synthesize and can remain stable under the effect of pH, system and photostability. In addition, it shows low cytotoxicity in cell imaging. And it can react with H2S highly selective in PBS or FBS solution, which would cause the obvious increase in fluorescence intensity. Therefore, the low-cost detection method for H2S is allowed for monitoring the quantity of H2S existed in the mitochondria.

• 出版日期2017-8
• 单位中国药科大学; 南京医科大学; 天然药物活性组分与药效国家重点实验室