Arachidonic acid triggers [Ca2+](i) increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release

作者：Paillamanque Joaquin; Sanchez Tusie Ana; Carmona Emerson M; Trevino Claudia L; Sandoval Carolina; Nualart Francisco; Osses Nelson; Reyes Juan G^*

来源：PLos One, 2017, 12(2): e0172128.

DOI：10.1371/journal.pone.0172128

Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca-2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca2+ release from intracellular Ca2+ stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.

• 出版日期2017-2-13