Determination of triamcinolone in human plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation

作者：Cesar Isabela Costa^*; Duarte Byrro Ricardo Martins; de Santana e Silva Cardoso Fabiana Fernandes; Mundim Iram Moreira; Teixeira Leonardo de Souza; de Sousa Weidson Carlo; Gomes Sandro Antonio; Bellorio Karini Bruno; Bretas Juliana Machado; Pianetti Gerson Antonio

来源：Journal of Mass Spectrometry, 2011, 46(3): 320-326.

DOI：10.1002/jms.1896

• triamcinolone
• nasal spray
• HPLC-ESI-MS/MS
• plasma
• pharmacokinetics

A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55 : 45). Selected reaction monitoring was performed using the transitions m/z 435 -> 415 and m/z 393 -> 373 to quantify triamcinolone acetonide and betamethasone, respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for triamcinolone acetonide. The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of triamcinolone acetonide in healthy volunteers, in a pharmacokinetic study with nasal spray formulation.

• 出版日期2011-3