N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO

作者：Jia, Guifang; Fu, Ye; Zhao, Xu; Dai, Qing; Zheng, Guanqun; Yang, Ying; Yi, Chengqi; Lindahl, Tomas; Pan, Tao; Yang, Yun-Gui; He, Chuan^*

来源：Nature Chemical Biology, 2011, 7(12): 885-887.

DOI：10.1038/NCHEMBIO.687

• GENOME-WIDE ASSOCIATION
• MESSENGER-RNA
• ADULT OBESITY
• DNA
• GENE
• ALKB
• RESIDUES
• REPAIR
• METHYLATION
• SPECIFICITY

We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.

• 出版日期2011-12
• 单位中国科学院北京基因组研究所