An improved surface passivation method for single-molecule studies

作者：Hua Boyang; Han Kyu Young; Zhou Ruobo; Kim Hajin; Shi Xinghua; Abeysirigunawardena Sanjaya C; Jain Ankur; Singh Digvijay; Aggarwal Vasudha; Woodson Sarah A; Ha Taekjip^*

来源：Nature Methods, 2014, 11(12): 1233-+.

DOI：10.1038/NMETH.3143

We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly (ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.

• 出版日期2014-12