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摘要
Objective: To explore the association of the CXCL12 gene polymorphism rs1801157 and the CXCR4 gene polymorphism rs2228014 and susceptibility to inflammatory bowel disease (IBD) in a Zhuang population from Guangxi, China. Methods: A case-control study was performed, which included intestinal tissue samples of 245 Zhuang patients with IBD in the experimental group and 202 healthy Zhuang subjects in the control group from January 2013 to February 2016. All patients and healthy subjects were from the Guangxi Zhuang Autonomous Region of China. Genomic DNA was extracted from intestinal tissue by the centrifugal column method. CXCL12 rs1801157 and CXCR4 rs2228014 polymorphisms were amplified by polymerase chain reaction (PCR), and then genotyped by restriction fragment length polymorphism (RFLP) analysis and sequencing. Results: The allele and genotype distributions of CXCL12 rs1801157 and CXCR4 rs2228014 were all in Hardy-Weinberg equilibrium for the IBD group and the control group. The genotypes of CXCL12 rs1801157 and CXCR4 rs2228014, including the homozygous mutant, heterozygous mutant and wild-type genotype, were found in both IBD patients and healthy controls. No significant differences were noted in genotype and allele frequencies of CXCL12 rs1801157 among UC/CD and control groups (P > 0.05). Differences in allele frequency between UC/CD patients and control group for CXCR4 rs2228014 were statistically significant (Chi(2) = 11.589, P = 0.001; Chi(2) = 14.365, P < 0.001). Conclusion: The CXCL12 gene rs1801157 polymorphism may not be associated with IBD in the Guangxi Zhuang population, but CXCR4 rs2228014 may be associated with IBD in the Guangxi Zhuang population.
- 出版日期2017
- 单位广西医科大学
