Dramatic improvement in genome assembly achieved using doubled-haploid genomes

作者：Zhang Hong; Tan Engkong; Suzuki Yutaka; Hirose Yusuke; Kinoshita Shigeharu; Okano Hideyuki; Kudoh Jun; Shimizu Atsushi; Saito Kazuyoshi; Watabe Shugo; Asakawa Shuichi^*

来源：Scientific Reports, 2014, 4(1): UNSP 6780.

DOI：10.1038/srep06780

Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold of doubled-haploid individuals, respectively, compared to the wildtype, indicating that the use of a doubled-haploid genome aids in accurate genome analysis.

• 出版日期2014-10-27