Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation

作者：Fernandes Lucas Matos; Mariani Alessandro Wasum; de Medeiros Israel Lopes; Samano Marcos Naoyuki; Abdalla Luis Gustavo; Correia Aristides Tadeu; Nepomuceno Natalia Aparecida; Canzian Mauro; Pego Fernandes Paulo Manuel^*

来源：Acta Cirurgica Brasileira, 2015, 30(5): 359-365.

DOI：10.1590/S0102-865020150050000008

• Organ Preservation Solutions
• Lung Injury
• Lung Transplantation

PURPOSE: To evaluate a new perfusate solution to be used for ex vivo lung perfusion.
METHODS: Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.
RESULTS: From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) 1,097 +/- 526g; Alternative Perfusion Solution (APS) 743 +/- 248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS 3.63 +/- 1.26; SG 2.06 +/- 0.28; p 0.009). No difference on PaO2 after EVLP (SG 498 +/- 37.53mmHg; APS 521 +/- 55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG 4.38 +/- 1.51; APS 4.50 +/- 1.77, p=0.881; apoptotic cells count after perfusion: SG 2.4 +/- 2.0 cells/mm(2); APS 4.8 +/- 6.9 cells/mm(2); p=0.361).
CONCLUSION: The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution r on lungs from rejected brain-dead donors.

• 出版日期2015-5