A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

作者：Perry Sarah L; Guha Sudipto; Pawate Ashtamurthy S; Bhaskarla Amrit; Agarwal Vinayak; Nair Satish K; Kenis Paul J A^*

来源：Lab on a Chip, 2013, 13(16): 3183-3187.

DOI：10.1039/c3lc50276g

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 angstrom via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

• 出版日期2013