Nanoparticle Delivery of the Bone Morphogenetic Protein 4 Gene to Adipose-Derived Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo

作者：Shi, Junjun; Zhang, Xin; Zhu, Jingxian; Pi, Yanbin; Hu, Xiaoqing; Zhou, Chunyan; Ao, Yingfang^*

来源：Arthroscopy-The Journal of Arthroscopic and Related Surgery, 2013, 29(12): 2001-U182.

DOI：10.1016/j.arthro.2013.09.076

• PLGA NANOPARTICLES
• KNEE ARTHROSCOPIES
• DIFFERENTIATION
• CHONDROGENESIS
• DEFECTS
• PROLIFERATION
• MICROFRACTURE
• EXPRESSION
• DENDRIMERS
• INJURIES

Purpose: To evaluate the effect of poly(lactic-co-glycolic acid) (PLGA) nanoparticles delivering pDC316-BMP4-EGFP plasmid into rabbit adipose-derived stem cells (ADSCs) in vitro and chondrogenesis of the bone morphogenetic protein 4 (BMP-4)-transfected ADSCs seeded onto poly(L-lactic-co-glycolic acid) (PLLGA) scaffold in a rabbit model. Methods: Cell viability and transfection efficiency of PLGA nanoparticles were measured by Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) and flow cytometry. The BMP-4 and chondrogenesis markers were detected by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Thirty rabbits (60 knees) with full-thickness cylinder articular cartilage defects (diameter, 4.5 mm; depth, 0.8 mm) on the femoral trochlea were divided into a group in which the BMP-4-transfected ADSCs were seeded onto PLLGA scaffold and implanted into the defects (group ABNP), a group with untransfected ADSCs seeded onto scaffold (group ABP), and a group with a scaffold without cells (group P). Outcomes were evaluated by histology, Rudert score, Pineda score, and scanning electronic microscopy by 2 blinded observers at weeks 6 and 12 postoperatively. Statistical analyses were performed with analysis of variance and the Kruskal-Wallis test. The statistical significance level was set at P < .05. Results: The expression of chondrogenesis-related genes and proteins was significantly increased in BMP-4-transfected ADSCs in vitro (P < .05). The cell viability was 79.86% +/- 5.04% after 24 hours. The transfection efficiency was 25.86% +/- 4.27% after 72 hours. Defects in group ABNP showed the best in vivo cartilage regeneration. At week 12, the Rudert scores in group ABNP (7.00 +/- 1.75) were better than those in group ABP (6.00 +/- 2.00) or group P (5.00 +/- 1.75) (P < .05), as were the Pineda scores (2.50 +/- 3.00, 5.00 +/- 2.00, and 6.00 +/- 1.75, respectively; P < .001). Conclusions: BMP-4 plasmid can be successfully delivered into ADSCs by PLGA nanoparticles and promoted in vitro chondrogenesis. When compared with the control cells, BMP-4-transfected ADSCs seeded onto PLLGA scaffold significantly improve in vivo chondrogenesis in a rabbit articular defect model.

• 出版日期2013-12
• 单位北京大学