Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing

作者：Komosinska Vassev Katarzyna^*; Olczyk Pawel; Winsz Szczotka Katarzyna; Kuznik Trocha Kornelia; Klimek Katarzyna; Olczyk Krystyna

来源：Clinical Chemistry and Laboratory Medicine, 2012, 50(3): 557-563.

DOI：10.1515/CCLM.2011.789

• ageing
• chondroitin sulphated glycosaminoglycans
• hyaluronan
• oxidative stress
• protein oxidation

Background: The authors studied the role of increased oxidative stress in the development of oxidative protein damage and extracellular matrix (ECM) components in ageing. The age- and gender-associated disturbances in connective tissue metabolism were evaluated by the plasma chondroitin sulphated glycosaminoglycans (CS-GAG) and non-sulphated GAG-hyaluronan (HA) measurements. Plasma concentration of advanced oxidation protein products (AOPP) was analysed in order to assess oxidative protein damage and evaluate the possible deleterious role of oxidative phenomenon on tissue proteoglycans%26apos; metabolism during the physiological ageing process. %26lt;br%26gt;Methods: Sulphated and non-sulphated GAGs as well as AOPP were quantified in plasma samples from 177 healthy volunteers. %26lt;br%26gt;Results: A linear age-related decline of plasma CS-GAG level was found in this study (r=-0.46; p%26lt;0.05). In contrast, HA concentrations rise gradually with age (r=0.44; p%26lt;0.05) in plasma samples. For both ECM components, the observed differences were not gender-specific. A strong age-dependent relationship has been shown in regard to AOPP. AOPP levels significantly increased with age (r=0.63; p%26lt;0.05), equally strongly in both men (r=0.69; p%26lt;0.05) and women (r=0.57; p%26lt;0.05) during physiological ageing. A significant correlation vas found between the concentrations of AOPP and both CS-GAG (r=-0.31; p%26lt;0.05) and HA (r=0.33; p%26lt;0.05). %26lt;br%26gt;Conclusions: Proceeding with age changes in the ECM are reflected by CS-GAG and HA plasma levels. Strong correlations between AOPP and ECM components indicate that oxidative stress targets protein and non-protein components of the connective tissue matrix during human ageing.

• 出版日期2012