Novel RNA polymerase II mutation suppresses transcriptional fidelity and oxidative stress sensitivity in rpb9 Delta yeast

作者：Koyama Hiroshi; Ueda Tomofumi; Ito Takahiro; Sekimizu Kazuhisa^*

来源：Genes to Cells, 2010, 15(2): 151-159.

DOI：10.1111/j.1365-2443.2009.01372.x

We previously reported that transcription elongation factor S-II and RNA polymerase II subunit Rpb9 maintain transcriptional fidelity and contribute to oxidative stress resistance in yeast. Here we examined whether other transcription elongation-related factors affect transcriptional fidelity in vivo. Among the 17 mutants of transcription elongation-related factors analyzed, most were not responsible for maintaining transcriptional fidelity. This finding indicates that transcriptional fidelity is controlled by a limited number of transcription elongation-related factors including S-II and Rpb9 and not by all transcription elongation-related factors. In contrast, by screening rpb9 Delta cell revertants for sensitivity to the oxidant menadione, we identified a novel mutation in RNA polymerase II, rpb1-G730D, which suppressed both reduced transcriptional fidelity and oxidative stress sensitivity. These findings suggest that the maintenance of transcriptional fidelity that is mediated by transcription machinery directly confers oxidative stress resistance.

• 出版日期2010-2