Mode decomposition and Lagrangian structures of the flow dynamics in orbitally shaken bioreactors

作者：Weheliye Weheliye Hashi; Cagney Neil; Rodriguez Gregorio; Micheletti Martina; Ducci Andrea^*

来源：Physics of Fluids, 2018, 30(3): 033603.

DOI：10.1063/1.5016305

In this study, two mode decomposition techniques were applied and compared to assess the flow dynamics in an orbital shaken bioreactor (OSB) of cylindrical geometry and flat bottom: proper orthogonal decomposition and dynamic mode decomposition. Particle Image Velocimetry (PIV) experiments were carried out for different operating conditions including fluid height, h, and shaker rotational speed, N. A detailed flow analysis is provided for conditions when the fluid and vessel motions are in-phase (Fr = 0.23) and out-of-phase (Fr = 0.47). PIV measurements in vertical and horizontal planes were combined to reconstruct low order models of the full 3D flow and to determine its Finite-Time Lyapunov Exponent (FTLE) within OSBs. The combined results from the mode decomposition and the FTLE fields provide a useful insight into the flow dynamics and Lagrangian coherent structures in OSBs and offer a valuable tool to optimise bioprocess design in terms of mixing and cell suspension. Published by AIP Publishing.

• 出版日期2018-3