Autologous Fat Processing Via the Revolve System: Quality and Quantity of Fat Retention Evaluated in an Animal Model

作者：Ansorge Heather; Garza Jaime R; McCormack Michael C; Leamy Patrick; Roesch Sana; Barere Aaron; Connor Jerome^*

来源：Aesthetic Surgery Journal, 2014, 34(3): 438-447.

DOI：10.1177/1090820X14524416

• autologous fat processing
• Revolve system
• fat retention
• animal model
• fat graft viability
• lipoaspiration
• adipose tissue
• centrifugation
• decantation

Background: Currently, fat graft viability and retention cannot be reliably predicted. The reasons for this variability are not fully understood, although fat processing has been implicated. %26lt;br%26gt;Objectives: The authors compare the in vitro quantity and in vivo fat retention from lipoaspirate processed by the Revolve system (LifeCell, Bridgewater, New Jersey) compared with centrifugation and decantation. %26lt;br%26gt;Methods: Ten patients were enrolled in this prospective study. Lipoaspirate from each patient was processed by each of 3 methods: decantation, centrifugation, and the Revolve system. Biochemical characteristics and free oil, adipose, and aqueous phases of the processed fats were determined. Fat grafts were implanted in nude mice; volume retention and quality of the fat grafts were evaluated after 28 days. Viability of retained fat was demonstrated by intact adipocytes and neovascularization on histology. %26lt;br%26gt;Results: Of the 10 patients, 9 were women and 1 was a man. Mean patient age was 40.7 8.9 years (range, 30-55 years). Fat tissue obtained from all methods had good physiological properties with neutral pH and isotonic salt concentrations. The Revolve system yielded significantly less blood cell debris, a higher percentage of adipose tissue, and a lower percentage of free oil compared with the other 2 methods. Fat tissue retention from Revolve samples was significantly higher (73.2%) than that from decanted samples (37.5%) and similar to that from centrifuged samples (67.7%). %26lt;br%26gt;Conclusions: The Revolve system produced physiologically compatible, preinjection fat with reduced contaminants and free oil in conjunction with high fat content. In an animal model, volume retention of Revolve-processed fat grafts was significantly greater than decanted samples. The Revolve system presents a fat-processing option that was less time-consuming, easier to use, and more efficient in this study than standard centrifugation or decantation.

• 出版日期2014-3