MafB negatively regulates RANKL-mediated osteoclast differentiation

作者：Kim Kabsun; Kim Jung Ha; Lee Junwon; Jin Hye Mi; Kook Hyun; Kim Kyung Keun; Lee Soo Young; Kim Nacksung^*

来源：Blood, 2007, 109(8): 3253-3259.

DOI：10.1182/blood-2006-09-048249

Receptor activator of nuclear factor kappa B ligand (RANKL) induces osteoclast formation from hematopoietic cells via regulation of various transcription factors. Here, we show that MafB negatively regulates RANKL-induced osteoclast differentiation. Expression levels of MafB are significantly reduced by RANKL during osteoclastogenesis. Overexpression of MafB in bone marrow-derived monocyte/macrophage lineage cells (BMMs) inhibits the formation of TRAP(+) multinuclear osteoclasts, but phagocytic activity of BMMs is retained. Furthermore, overexpression of MafB in BMMs attenuates the gene induction of NFATc1 and osteoclast-associated receptor (OSCAR) during RANKL-mediated osteoclastogenesis. In addition, MafB proteins interfere with the DNA-binding ability of c-Fos, Mitf, and NFATc1, inhibiting their transactivation of NFATc1 and OSCAR. Furthermore, reduced expression of MafB by RNAi enhances osteoclastogenesis and increases expression of NFATc1 and OSCAR. Taken together, our results suggest that MafB can act as an important modulator of RANKL-mediated osteoclastogenesis.

• 出版日期2007-4-15
• 单位河北医科大学