Attenuation of TRPV1 by AMG-517 after nerve injury promotes peripheral axonal regeneration in rats

作者：Bai, Juan; Liu, Fu; Wu, Li-Fei; Wang, Ya-Fang; Li, Xia-Qing^*

来源：Molecular Pain, 2018, 14: 1744806918777614.

DOI：10.1177/1744806918777614

• Peripheral nerve injury and regeneration
• transient receptor potential cation channel subfamily member V1
• calcitonin gene-related peptide
• glial fibrillary acidic protein
• growth-associated protein-43

Aims: The main objective was to investigate the effects of the transient receptor potential cation channel subfamily V member 1 (TRPV1) on nerve regeneration following sciatic transection injury by functional blockage of TRPV1 using AMG-517, a specific blocker of TRPV1.
Methods: AMG-517 was injected into the area surrounding ipsilateral lumbar dorsal root ganglia 30 min after unilateral sciatic nerve transection. The number of sciatic axons and the expression of growth-associated protein-43 (GAP-43) and glial fibrillary acidic protein was examined using semithin sections, Western blot, and immunofluorescence analyses.
Results: Blockage of TRPV1 with AMG-517 markedly promoted axonal regeneration, especially at two weeks after sciatic injury; the number of axons was similar to the uninjured control group. After sciatic nerve transection, expression of glial fibrillary acidic protein was decreased and GAP-43 was increased at the proximal stump. However, the expression of both glial fibrillary acidic protein and GAP-43 increased significantly in AMG-517-treated groups.
Conclusions: TRPV1 may be an important therapeutic target to promote peripheral nerve regeneration after injury.

• 出版日期2018-6-8
• 单位山西医科大学