A simplified method to assess affinity of insulin autoantibodies

作者：Achenbach Peter^*; Guo Liang Hao; Gick Claudia; Adler Kerstin; Krause Stephanie; Bonifacio Ezio; Colman Peter G; Ziegler Anette G

来源：Clinical Immunology, 2010, 137(3): 415-421.

DOI：10.1016/j.clim.2010.09.002

• Insulin autoantibody
• Affinity
• Type 1 diabetes
• Prediction

Insulin autoantibodies (IAA) precede type 1 diabetes, but not all IAA positive children develop other islet autoantibodies and disease Diabetes risk can be stratified by laborious IAA affinity measurement using competition with multiple ligand concentrations Here, we identify a single competitor concentration that discriminates low and high affinity IAA Discrimination was achieved among 122 IAA positive sera using 7 0 nM competitor which is 54 fold that of the assay radioligand concentration Relative binding <60% at this competitor concentration identified all 85 sera with affinities >= 1 0x 10(8) L/mol and none with lower affinities (P<0 0001), and 45 (96%) of 47 multiple Islet autoantibody positive sera (P<0 0001) IAA competition was further tested in a second set of 119 IAA positive sera Of these, 99 fulfilled high affinity competition criteria of <60% relative binding at 7 0 nM competitor including 89

• 出版日期2010-12