A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

作者：Tanaka Niina; Saitoh Hisato^*

来源：Bioscience Biotechnology and Biochemistry, 2010, 74(6): 1302-1305.

DOI：10.1271/bbb.1000821

• post-translational modification
• SUMO
• protein interaction
• histone methyltransferase
• chromatin

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

• 出版日期2010-6