A genetic linkage map of marine shrimp Penaeus (Fenneropenaeus) chinensis based on AFLP, SSR, and RAPD markers

作者：Liu Bo; Wang Qingyin; Li Jian^*; Liu Ping; He Yuying

来源：Chinese Journal of Oceanology and Limnology, 2010, 28(4): 815-825.

DOI：10.1007/s00343-010-9915-3

• AFLP
• linkage map
• Penaeus (Fenneropenaeus) chinensis
• RAPD
• SSR

The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a "Yellow Sea No. 1" male to produce the first filial generation (F(1)) 100 F(2) full-sib progeny from brother-sister crosses between F(1) families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.

• 出版日期2010-7
• 单位中国海洋大学; 中国水产科学研究院