Soybean Bowman-Birk Protease Inhibitor (BBI): Identification of the Mechanisms of BBI Suppressive Effect on Growth of Two Adenocarcinoma Cell Lines: AGS and HT29

作者：Fereidunian Amirhossein; Sadeghalvad Mona; Oscoie Maryam Omidi; Mostafaie Ali^*

来源：Archives of Medical Research, 2014, 45(6): 455-461.

DOI：10.1016/j.arcmed.2014.07.001

• Bowman-Birk protease inhibitor
• Vascular endothelial growth factor
• Matrix metalloproteinase
• Angiogenesis
• Purification

Background and Aims. Bowman-Birk protease inhibitor (BBI) has been well known to suppress the emergence and progression of different cancers. In the present study, the mechanisms by which BBI alters cancers have been addressed. To reach this goal, the effects of BBI on proliferation of and VEGF secretion by two cell lines (AGS: gastric adenocarcinoma and HT-29: colorectal adenocarcinoma) and also BBI effect on MMP-2 and 9 synthesis/secretion by AGS cells was evaluated.
Methods. ELISA method was used to assess VEGF concentration and gelatin zymography was used to address MMP-2 and 9 production/excretion.
Results. BBI had powerful inhibitory effect on proliferation and VEGF secretion by both cell lines. In addition, inhibition of MMP-2 and MMP-9 secreted by AGS cells suggests BBI as a potent inhibitor of gastric cancer progression. On the other hand, the results indicated that inhibition of MMP-2, MMP-9 and VEGF secretion is one of the mechanisms of anti-angiogenic effect of BBI.
Conclusion. BBI expresses powerful suppressive effect on tumor progression of two prevalent cancers: gastric adenocarcinoma and colorectal adenocarcinoma.

• 出版日期2014-8