Soy whey based medium for optimized phytase activity in Saccharomyces cerevisiae MTCC 5421 and alpha-D-galactosidase and antibacterial activities in Lactobacillus plantarum MTCC 5422 by response surface methodology

作者：Roopashri Arekal N; Varadaraj Mandyam C^*

来源：Journal of Food Science and Technology-Mysore, 2014, 51(3): 519-526.

DOI：10.1007/s13197-011-0527-5

• Lactobacillus plantarum
• Saccharomyces cerevisiae
• alpha-D-galactosidase
• Phytase
• Antibacterial
• Soy whey based medium

Soy whey based growth medium for yeast enabled the native isolate of Saccharomyces cerevisiae MTCC 5421 to produce 198 U/ml of phytase activity against that of 135 U/ml in potato dextrose broth. In the same medium for lactic acid bacteria, the isolate of Lactobacillus plantarum MTCC 5422 was able to elaborate alpha-D-galactosidase activity of 10.6 U/ml in comparison to 6 U/ml in Lactobacillus MRS broth. Using this formulated medium, a central composite experimental design based on 5 variables of 3 factors namely incubation temperature, pH level and incubation period showed that S. cerevisiae could produce 200 U/ml of phytase in 36 h at 30 A degrees C and pH 3.5. Similarly, L. plantarum in 33 h at 37 A degrees C and pH 6.6 exhibited 14.2 U/ml of alpha-D-galactosidase activity, whereas the antibacterial activity of 14.4 AU/ml against Bacillus cereus was evident in 42 h at 42 A degrees C and pH 6.0. The phytase and antibacterial activity units visualized in response surface plots were more or less close to those obtained with the experimental design treatments.

• 出版日期2014-3