Acute starvation in C57BL/6J mice increases myocardial UCP2 and UCP3 protein expression levels and decreases mitochondrial bio-energetic function

作者：Wang Chun Ming; Almsherqi Zakaria A; McLachlan Craig S; Matthews Slade; Ramachandran Malarmathy; Tay Stacey K H; Deng Yuru^*

来源：Stress-The International Journal on the Biology of Stress, 2011, 14(1): 66-72.

DOI：10.3109/10253890.2010.506931

• Cardiac myocytes
• mitochondrial bio-energetics
• oxidative stress
• starvation
• uncoupling protein

Associations between uncoupling protein (UCP) expression and functional changes in myocardial mitochondrial bio-energetics have not been well studied during periods of starvation stress. Our aim was to study the effects of acute starvation, for 24 or 48 h, on combined cardiac mitochondrial function and UCP expression in mice. Isolated heart mitochondria from female mice starved for 48 h compared to that from mice fed revealed a significantly (p < 0.05) decreased adenosine diphosphate-to-oxygen ratio, a significantly increased proton leak and an increased GTP inhibition on palmitic acid-induced state 4 oxygen consumption (p < 0.05). These bio-energetic functional changes were associated with increases in mitochondrial UCP2 and UCP3 protein expression. In conclusion, our findings suggest that increased UCP2 and UCP3 levels may contribute to decreased myocardial mitochondrial bio-energetic function due to starvation.

• 出版日期2011-1