Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR

作者：Huangfu, Heping^*; Xu, Wenbo; Wang, Hongkui; Dong, Qing; Guo, Hongwei; Sun, Yanting; Li, Yunxia; Gao, Wenwen; Wang, Wuqing; Zhang, Jia; Shi, Jingke; Pan, Haochun; Li, Congcong; Wang, Linkang

来源：Avian Pathology, 2018, 47(3): 245-252.

DOI：10.1080/03079457.2017.1416590

• TaqMan fluorescent quantitative PCR
• probe
• detection
• poultry
• prevalence

To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis. A comparison of the detection results in 160 clinical swab samples showed that the detection rate (54.4%) of the qPCR for G. anatis was better than that of two conventional methods: gyrB gene-based qPCR for G. anatis (51.9%) and culture-based identification (34.4%). G. anatis was detected in layer chicken (77.3%), Silkie chicken (72.7%), and duck (27.1%) with relatively high detection rates, whereas dove (8.8%) and quail (3.0%) showed lower detection rates, indicating the different prevalence of G. anatis in different fowl species.

• 出版日期2018
• 单位河南大学; 河南牧业经济学院