siRNA specific to pdx-1 disturbed the formation of the islet in early zebrafish embryos

作者：Chen Shen; Huang Jintao; Yuan Guangming; Chen Qian; Huang Nannan; Xie Fukang^*

来源：Journal of Huazhong University of Science and Technology-Medical Sciences, 2007, 27(6): 639-642.

DOI：10.1007/s11596-007-0604-1

• pancreas
• pancreatic development
• pdx-1
• siRNA
• zebrafish

Pdx-1, an important transcription factor highlighting in the early pancreatic development, islet functions and pancreatic disorders, needs to be more investigated in zebrafish, and siRNA is still seldom applied in zebrafish embryo-related research. Our aim was to explore the role of pdx-1 in pancreatic development of zebrafish embryos by using siRNA approach. Microinjection, reverse transcriptase-PCR (RT-PCR), in situ hybridization and immunofluorescent staining were used in this research, and the morphology of the islet in normal zebrafish embryos, and in those treated with the siRNA specific to pdx-1 (siPDX-1) or siGFP was observed and compared. The expression of pdx-1 was detected in the stages of 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, 16-hour by RT-PCT. The in situ hybridization and immunofluorescent staining results showed that siPDX-1 disturbed the formation of the islet in zebrafish embryos. Pdx-1 played multiple roles in maintaining the phenotype of the islet during embryogenesis in zebrafish.

• 出版日期2007-12
• 单位中山大学