Stable hydrogen isotope measurement of archaeal ether-bound hydrocarbons

作者：Kaneko Masanori; Kitajima Fumio; Naraoka Hiroshi^*

来源：Organic Geochemistry, 2011, 42(2): 166-172.

DOI：10.1016/j.orggeochem.2010.11.002

Compound-specific hydrogen isotope analysis of ether-bound isoprenoid hydrocarbons from archaeal membranes has been developed using chemical degradation and gas chromatography/pyrolysis/isotope ratio mass spectrometry. The ether-bound hydrocarbons are quantitatively converted to saturated hydrocarbons by cleavage of ether bonds with HI followed by H(2) reduction in the presence of PtO(2). The delta D value of ether-bound hydrocarbon moieties are corrected by way of isotopic mass balance calculation for the hydrogen incorporated during the hydrogenation. The method was successfully applied to determination of the delta D values of biphytane moieties in glycerol dialkyl glycerol tetraethers (GDGTs) from a Sulfolobus sp. culture and a marine sediment.

• 出版日期2011-2