Does nonylphenol promote the growth of uterine fibroids?

作者：Shen, Yang^*; Ren, Mu-Lan; Feng, Xu; Gao, Yong-Xing; Xu, Qian; Cai, Yun-Lang

来源：European Journal of Obstetrics & Gynecology and Reproductive Biology, 2014, 178: 134-137.

DOI：10.1016/j.ejogrb.2014.04.038

• Uterine leiomyoma
• Environmental oestrogen
• Nonylphenol
• ER alpha
• IGF-1
• VEGF

Objective: To study the effect and mechanism of action of nonylphenol (NP), an environmental oestrogen, on uterine leiomyoma (UL) cells. @@@ Methods: Primary culture and subculture of human UL cells, identified as smooth muscle cells by immunocytochemical staining with a monoclonal anti-alpha-smooth muscle actin antibody, were performed. The viability of cells treated with various concentrations of NP for 24, 48 and 72 h was determined by CCK-8 assay. mRNA expression of oestrogen receptor alpha (ER alpha), insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was detected using real-time quantitative polymerase chain reaction, and protein expression was detected using Western blot analysis for all groups. @@@ Results: NP promoted the growth of UL cells and expression of ER alpha, IGF-1 and VEGF; this was positively correlated with the concentration and duration of NP treatment. @@@ Conclusion: NP promotes the growth of UL cells. The mechanism of action appears to be over-expression of IGF-1 and VEGF, up-regulated by ER alpha, resulting in the growth of UL cells.

• 出版日期2014-7
• 单位东南大学