科研之友
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摘要
We observed intracellular transportation by using surface-enhanced Raman scattering (SERS) from gold nanoparticles moving in the cytosol. Simultaneous detection of the nanoparticle three-dimensional position and SERS spectra presents molecular vibrational modes and contains large amounts of information about bimolecular events. In order to determine the meaningful temporal spectral changes associated with specific intracellular events or functions, we analyzed the time series Raman spectra, considering both the motion of the gold nanoparticle and also the time-varying spectra, revealing that some molecular vibrational modes showed strong correlation with the gold nanoparticle motion. This type of time-space-spectrum variable analysis will enable us to visualize molecular dynamics such as dissociation of proteins in biological reactions. In particular, our approach enables us to study molecules which should be associated with the transportation process such as vesicle transport, nuclear entry and protein diffusion.
- 出版日期2015-11
