A Combination of (omega-3) Polyunsaturated Fatty Acids, Polyphenols and L-Carnitine Reduces the Plasma Lipid Levels and Increases the Expression of Genes Involved in Fatty Acid Oxidation in Human Peripheral Blood Mononuclear Cells and HepG2 Cells

作者：Radler Ulla; Stangl Herbert; Lechner Sigrid; Lienbacher Gerhard; Krepp Rainer; Zeller Eduard; Brachinger Martin; Eller Berndl Doris; Fischer Andreas; Anzur Christian; Schoerg Gerhard; Mascher Daniel; Laschan Claudia; Anderwald Christian; Lohninger Alfred^*

来源：Annals of Nutrition and Metabolism, 2011, 58(2): 133-140.

DOI：10.1159/000327150

• omega-3 polyunsaturated fatty acids
• Carnitine
• Polyphenols
• Plasma lipids
• Peripheral blood mononuclear cells
• HepG2 cells
• Gene expression

Background: Hyperlipidemia and obesity are associated with metabolic syndrome and increased risk in developing diabetes and cardiovascular disease. Nutritional supplements, e. g. L-carnitine and polyunsaturated fatty acids (PUFAs), exert lipid-lowering effects. Hence, the hypothesis that dietetic intervention reduces plasma lipid levels and metabolic enzymes in overweight hyperlipidemic subjects was tested. Subjects and Methods: In a prospective placebo-controlled double-blind study in 22 moderately hyperlipidemic obese humans consuming low-fat yoghurt enriched with a combination of low-dose PUFAs, polyphenols and L-carnitine (PPC) twice a day for 12 weeks were compared to 20 matching participants ingesting low-fat yoghurt. The effects on plasma lipids and expression of enzymes involved in regulation of fatty acid oxidation in peripheral blood mononuclear cells (PBMCs) and HepG2 cells were evaluated. Results: PPC consumption led to significantly reduced plasma free fatty acid (-29%) and triglyceride (-24%) concentrations (each p < 0.05). PPC application increased significantly peroxisome proliferator-activated receptor alpha (PPAR alpha) mRNA abundances and those of PPAR alpha target genes (carnitine palmitoyltransferases-1, CPT1A and CPT1B, carnitine acetyltransferase and organic cation transporter 2; each p < 0.05) in PBMCs. In controls, plasma lipid levels and PBMC gene expression did not change. These findings were substantiated by the results of cell culture experiments in HepG2 cells. Conclusion: Supplementation of PPC had marked lipid-lowering effects and PBMC gene expression profiles seemed to reflect nutrition-related metabolic changes.

• 出版日期2011
• 单位河北医科大学