A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus polymyxa EJS-3 by macroporous resin

作者：Liu, Jun; Luo, Jianguang; Sun, Yi; Ye, Hong; Lu, Zhaoxin; Zeng, Xiaoxiong^*

来源：Bioresource Technology, 2010, 101(15): 6077-6083.

DOI：10.1016/j.biortech.2010.03.019

• Adsorption
• Decoloration
• Deproteinization
• Levan
• Macroporous resin

A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 degrees C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5 mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P. polymyxa EJS-3.

• 出版日期2010-8
• 单位南京农业大学