Molecular identification of macroscopic cysts of Sarcocystis in sheep in Babol, in the north of Iran

作者：Hatami A; Rashno Z^*; Hamidinejat H; Youssefi M R

来源：Tropical Biomedicine, 2017, 34(2): 405-411.

Sarcocystis is one of the most prevalent parasite in domestic animals in the world. In this study, we examined 50 macroscopic cysts in sheep muscles from Babol, in the north of Iran. Genomic DNA extraction and polymerase chain reaction (PCR) were performed to amplify a 609bp length based on 18S rRNA gene. The results of restriction of AvaI, Hind II, TaqI and EcoRI enzymes demonstrated that all the samples were Sarcocystis gigantea. The results of this study supports the importance of molecular techniques for characterization of Sarcocystis species when valid preventive programs for identification and source of infection and progression of immunological diagnosis strategies are needed.

• 出版日期2017-6