A mobile endocytic network connects clathrinin-dependent receptor endocytosis to recycling and promotes T cell activation

作者：Compeer Ewoud B; Kraus Felix; Ecker Manuela; Redpath Gregory; Amiezer Mayan; Rother Nils; Nicovich Philip R; Kapoor Kaushik Natasha; Deng Qiji; Samson Guerric P B; Yang Zhengmin; Lou Jieqiong; Carnell Michael; Vartoukian Haig; Gaus Katharina; Rossy Jeremie^*

来源：Nature Communications, 2018, 9(1): 1597.

DOI：10.1038/s41467-018-04088-w

Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that control the organization of recycling endosomes and how they connect to receptor endocytosis. Here, we follow the endocytic journey of the T cell receptor (TCR), from internalization at the plasma membrane to recycling back to the immunological synapse. We show that TCR triggering leads to its rapid uptake through a clathrin-independent pathway. Immediately after internalization, TCR is incorporated into a mobile and long-lived endocytic network demarked by the membrane-organizing proteins flotillins. Although flotillins are not required for TCR internalization, they are necessary for its recycling to the immunological synapse. We further show that flotillins are essential for T cell activation, supporting TCR nanoscale organization and signaling.

• 出版日期2018-4-23