Analysis of Differentially Expressed Proteome in Urinary Exosome from Non-small Cell Lung Cancer Patients

作者：Dai Yi; Zhang Yuan; Qiu Feng; Li Yan Yan; Qiu Zong Yin^*

来源：Chinese Journal of Analytical Chemistry, 2010, 38(3): 325-331.

DOI：10.1016/S1872-2040(09)60030-X

• Exosome
• Non-small cell lung cancer
• Sodium dodecylsulfonate-polyacrylate gel electrophoresis
• High performance liquid chromatography-chip-tandem mass spectrometry

Urine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer (NSCLC) patients' urine. For proteomic analysis of urinary exosome, 1D sodium dodecylsulfonate-polyacrylate gel electrophoresis(SDS-PAGE) was carries out and cut the gel 31 kDa - 20 kDa bands in normal group and disease group's. These gel blocks were subjected to in-gel trypsinization, and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISS-PORT. The difference expression proteins were found in urinary exosome from NSCLC patients, including three fragment of the immunoglobulin kappa, two kinds of Ras related proteins, glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1.

• 出版日期2010-3
• 单位重庆医科大学