Anti-Toxoplasma Activity of 2-(Naphthalene-2-gamma lthiol)-1H Indole

作者：Asgari Qasem^*; Keshavarz Hossein; Rezaeian Mostafa; Sadeghpour Hossein; Miri Ramin; Motazedian Mohammad Hossein

来源：Iranian Journal of Parasitology, 2015, 10(2): 171-180.

• Toxoplasma gondii
• 2-(naphthalene-2-ylthio)-1H-indole
• Viability
• Infectivity
• Immunity

Background: This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1H-indole.
Methods: Tachyzoites of RH strain were incubated in various concentrations of 2( naphthalene-2-ylthio)-1H-indole (25-800 mu M) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS). To evaluate the infectivity, the tachyzoites exposed to the different concentrations of the compound were inoculated to 10 BALB/c mice groups. For Control, parasites exposed to DMSO (0.2% v/v) were also intraperitoneally inoculated into two groups of mice. The immunity of the exposed tachyzoites was evaluated by inoculation of the naive parasite to the survived mice.
Results: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 mu mol. The longevity of mice was dose dependent. Five mice out of group 400 mu mol and 3 out of group 800 mu mol showed immunization to the parasite.
Conclusion: Our findings demonstrated the toxoplasmocidal activity of the compound. The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

• 出版日期2015-6