Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region

作者：Takashima S; Iikura H; Nakamura A; Masaki H; Uozumi T

来源：FEMS Microbiology Letters, 1996, 145(3): 361-366.

DOI：10.1016/s0378-1097(96)00432-6

• carbon catabolite repression
• cellobiohydrolase
• Cre1
• Trichoderma reesei
• Zinc finger domain

A 1.5-kb XbaI-SacII fragment containing the upstream region of the Trichoderma reesei cellobiohydrolase I gene (cbhl) has been sequenced. The 1.5-kb fragment contains eight 6-bp sites having an identical or similar sequence to the consensus sequence for binding a catabolite repressor, Aspergillus nidulans CreA. Results of binding assays with the maltose-binding protein::Cre1(10-131) fusion protein (Cre1 is a catabolite repressor of T. reesei) and the cbh1 upstream region revealed that a 504-bp XbaI-NspV fragment (nucleotide position -1496 to -993) bearing three 6-bp sites, A1, A2, and A3, and a 356-bp NspV-MunI fragment (nucleotide position -994 to -639) bearing three 6-bp sites, B1, B2, and B3, were shifted in the electrophoretic mobility shift assay. DNase I footprinting experiments showed that the 6-bp sites A2, B1, B2, and B3 were protected from DNase I digestion.

• 出版日期1996-12-15