Mitochondrial ND-1 gene-specific primer polymerase chain reaction to determine mice contamination in meatball

作者：Raharjo T. J.^*; Nuryanti I; Patria F. P.; Swasono R. T.

来源：International Food Research Journal, 2018, 25(2): 638-642.

• Mitochondrial DNA
• Mice
• Specific primer-PCR
• Meat products

A specificity method to detect mice meat contamination in beef meatballs using specific primer-polymerase chain reaction (PCR) technique has been developed. The primer ND1-P1 primers were designed using primer-BLAST software using mtDNA of mice as a template. The Primer ND1-P1 forward (5'-CGGCATCCTACAACCATTTGC-3') and reverse (5'-CGGCTCGTAAAGC-TCCGAA-3') was able to amplify a 294 bp fragment of ND1 gene in mice mtDNA. The primers have been proven precise with only amplify the target fragment in mice meatball but not in another meatball including beef meatball, chicken meatball, pork meatball, horse meatball, and goat meatball. The present of mice meat in meatballs can be detected at a concentration as low as 5% (w/w). The ND1-P1 primer is potentially used as a specific marker for detection of mice meat in the meat products.

• 出版日期2018-4