A low pressure on-chip injection strategy for high-performance chip-based chromatography

作者：Thurmann S; Dittmar A; Belder D^*

来源：Journal of Chromatography A, 2014, 1340: 59-67.

DOI：10.1016/j.chroma.2014.03.009

• Miniaturization
• Chip-chromatography
• Injection
• Microfluidics
• Fluorescence
• PAH

A microfluidic device for high-performance liquid chromatography is presented. The borosilicate glass chip includes a common cross-channel injector and a column chamber. The column chamber comprises two constrictions (10 mu m) to retain the chromatographic particles via the keystone effect. A packing channel was utilized to introduce the particulate material (3-mu m). This channel has been sealed permanently via laser-assisted photopolymerization avoiding dead volumes within the chromatographic pathway. A sampling strategy is presented enabling the directed definition of the sample plug on-chip. The injection process is studied via video microscopy and chromatographic separations of test solutes with fluorescence detection. Our approach, based on on-chip flow splitting, allows the usage of traditional, economic and robust HPLC instrumentation avoiding the need for sophisticated nano-flow equipment. The performance of the chip device and the injection process was demonstrated by reversed-phase chromatograph

• 出版日期2014-5-2